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Protein Crystallography Forum Discuss research on protein crystallography and crystalization. Protein Crystallography Forum.


I am new to this forum and would like to ask for some advice

I am new to this forum and would like to ask for some advice - Protein Crystallography Forum

I am new to this forum and would like to ask for some advice - Discuss research on protein crystallography and crystalization. Protein Crystallography Forum.


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Old 06-28-2010, 07:35 PM
Pipette Filler
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Default I am new to this forum and would like to ask for some advice



Hi Everyone,

I am new to the forum and need some advice,

I am trying to prepare a grid screen with an automated instrument; I am preparing a 100mM HEPES pH gradient from pH 6.8 to 8.2 across 6 wells of a 24 well plate,

What recommendation does anyone have for the appropriate base to get this gradient ?

Running a TRIS gradient with the robot works great from pH 7 through 9 so I am confident of the instrument's ability but for the HEPES gradient, I cannot seem to find a suitable base to meet this,

I tried a low NaOH solution but was concerned that I would dilute out the 100mM HEPES,

Can anyone offer assistance ?

I am more of an automation specialist rather than a biologist,

thanks

Automation101
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