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Protein Crystallography Forum Discuss research on protein crystallography and crystalization. Protein Crystallography Forum.


Glycosylations

Glycosylations - Protein Crystallography Forum

Glycosylations - Discuss research on protein crystallography and crystalization. Protein Crystallography Forum.


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Old 06-16-2009, 07:59 AM
Pipette Filler
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Default Glycosylations



I have a protein with four glycosylation sites, 3 N-linked and 1 O-linked. I have tried to remove the N-linked residues with PNGase F and Endo H without success. Does anyone know of better enzymes or better methods to deglycosylate native proteins?
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Old 06-30-2009, 02:37 AM
Pipette Filler
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Default Re: Glycosylations

Hey actually my protein has 2 glycosylation sites , one of which we mutated and the other one we are trying to digest it with PNGase F and Endo H but I don't know the result right now.......what exact procedure and unit you use to see the enzyme activity. I know PNGaseF is a kind of control but Endo H deglycosylate the protein to some extent.
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