| | with tag or without tag..
You have two choices whether to crystallize your protein with or without the tag. Both of them might work. If you decide to remove it, then totally remove it from your protein. In my opinion, you can crystallize your protein including the tag. But it would incorporated together with your protein structure, thus it would not show the real structure of your protein. Is that your protein including the tag is 18kDa? The size is small. In this case, you have to make sure that you have enough and high protein content..Because small size of protein will hardly crystallize if it have low protein content..
Last edited by miss deane; 03-11-2009 at 03:01 PM.