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ميدان فارسى. Persian Molecular Biology ميدان فارسى. Persian Iranian Molecular Biology Forums. Speak Farsi in this forum for Iran.


آر.ان.آ

آر.ان.آ - ميدان فارسى. Persian Molecular Biology

آر.ان.آ - ميدان فارسى. Persian Iranian Molecular Biology Forums. Speak Farsi in this forum for Iran.


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  #1  
Old 07-09-2008, 03:51 AM
Pipette Filler
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Default آر.ان.آ



سلام چقدر خوبه که می شه اینجا فارسی صحبت کرد این خیلی با ارزشه.من روی(آر.ان.آ) کار می کنم یه مشکلی دارم نمونه های من بافتهای طحال هستن (موش) که حدود 6 سال در فریزر -70 نگهداری شدند.من با کیت استخراج (آر.ان.آ)کمپانی(رش)استخراج انجام دادم . بعضی از نمونه ها خیلی خوب کار می کنند ولی بعضیهاشون
rt-pcr
اونها کار نمیکنه این نمونه ها رو با ترایزول هم استخراج کردم ولی باز هم همین نتیجه رو داد.نمیددونم باید چیکار کنم.نمونه هام خیلی مهم هستن.خیلی ممنون میشم اگه کسی راهنمایی به نظرش میرسه کمکم کنه.
شمیلا
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Old 05-29-2010, 07:18 AM
Pipette Filler
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Default Re: آر.ان.آ

fjpsdf sd dfas sda f
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Old 07-26-2010, 09:46 AM
ahs ahs is offline
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Default Re: آر.ان.آ

Hi, I love to write persian, but I do not have keyboard, sorry for English reply.
Have you tried to dilute your samples? If not, it is worth trying at least 10x dilution. Because dilution may decrease inhibitors effect (samples or amplicon).
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