You have isolated a decapeptide called FP which has anticancer activity.

[Gloria]

Determine the sequence of the peptide? From the following information. One cycle of demand degradation of intact FP yields 2 mole of PTH-aspartate per mole of FP. Treatment of a solution of FP with 2-ME followed by the addition of trypsin yields three peptides (Ala, Cys, Phe) (Arg, Asp) (Asp, Cys, Gly, Met, Phe) the intact (Ala, Cys, Phe) peptide yields PTH-cysteine in the first cycle of demand degradation. Treatment of 1 mol of FP with carboxypeptidase (cleaves at C-terminus of all residues) yields 2 mol of phenylalanine. Treatment of intact pentapeptide (Asp, Cys, Gly, Met, Phe) with BrCN yields two peptides with composition (homoserine lactose, Asp) and (Cys, Gly, Phe) the (Cys, Gly, Phe) peptide yields PTH-glycine in the first cycle of demand degradation