At what pH will the following peptides be hard to separate by...
...electrophoresis: Lys-his-pro-asp-ala-arg-glu-phe? At what pH will the following peptides be hard to separate by electrophoresis:
and why ? explain please ?
Re: At what pH will the following peptides be hard to separate by...
Ans is c- pH 2.3
At pH 2.3 the following peptides be hard to separate by electrophoresis:
Because at this pH Side chain of aspartic acid remains protonated and not contribute to generate -ve charge so both the peptide having same Net charge.
While in all other pH value peptide Lys-his-pro-asp-ala-arg-glu-phe-ser having one extra -ve charge because of aspartic side chain, SO it separated from other peptide Lys-his-pro-asn-ala-arg-glu-phe-ser as it having Asparagine which having amide side group so not contribute any extra charge at any pH.
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