Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Proteomics Forum > Peptide Forum
Register Search Today's Posts Mark Forums Read

Peptide Forum Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


What is a peptide library and what are pooled immunoglobulins?

What is a peptide library and what are pooled immunoglobulins? - Peptide Forum

What is a peptide library and what are pooled immunoglobulins? - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-20-2012, 07:15 PM
Pipette Filler
Points: 6, Level: 1 Points: 6, Level: 1 Points: 6, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2012
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default What is a peptide library and what are pooled immunoglobulins?



I am reading a paper about autoimmune pancreatitis and it says that they screened a peptide library that can show peptides on the surface of E.coli with pooled immunoglobuins obtain from 20 patients with autoimmune pancreatitis.
Reply With Quote
  #2  
Old 11-28-2012, 02:35 PM
Pipette Filler
Points: 77, Level: 1 Points: 77, Level: 1 Points: 77, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2012
Location: Buenos Aires, Argentina
Posts: 3
Thanks: 1
Thanked 0 Times in 0 Posts
Default Re: What is a peptide library and what are pooled immunoglobulins?

A peptide library is an easy way to obtain thousands of peptides. They can be as long as you want, and been made of so many different aminoacids as you think neccesary (obviuosly you need to understand that if you make a giant library it would be very diffivult to manipulate it). The library is made by the method DCR (divide-couple-recombine). The Fmoc synthesis is the most use.
When u have the library u can screen with a lebeled protein, to see if some peptides have affinity for your protein.

If u have any doubt let me know.

Juan Manuel
Reply With Quote
Reply

Tags
immunoglobulins , library , peptide , pooled


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 01:52 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11147 seconds with 15 queries