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# Finding net charge on a peptide?

## Finding net charge on a peptide? - Peptide Forum

### Finding net charge on a peptide? - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.

#1
10-07-2012, 01:19 AM
Pipette Filler

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Finding net charge on a peptide?

Consider the net charge on the peptide AAAADEHKRAAA dissolved in an aqueous solution. Suppose the pH is raised from 2 to 8.5. Ignoring the charges that may exist on the N- and C-termini, what is the correct charge on the peptide?

a. from +2 to +5
b. from +3 to +5
c. from +3 to 0.
d. from 0 to +2

Thanks so much!
#2
10-08-2012, 03:44 AM

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Re: Finding net charge on a peptide?

C) +3 to 0

At pH 2 Side chain Asp (D) and Glu (E) having 0Charge while His(H), Lys(K),Arg (R) having +1 Charge each so total +2 charge .

Net charge at pH 2 is +3 (Ignoring N and C termini amino acid)

At pH 8.5 Side chain Asp (D) and Glu (E) having -1 Charge each so total -2 Charge while His(H) having 0 charge , while Lys(K),Arg (R) having +1 Charge each so total +2 charge

Net charge at pH 8.5 is 0 (+2 - 2) (Ignoring N and C termini amino acid)
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Kunal Pandya

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