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You are characterizing a newly isolated peptide which serves as a hormone.?

You are characterizing a newly isolated peptide which serves as a hormone.? - Peptide Forum

You are characterizing a newly isolated peptide which serves as a hormone.? - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


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Old 09-18-2012, 08:16 PM
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Default You are characterizing a newly isolated peptide which serves as a hormone.?



In addition to other amino acids, the hormone is found to contain a single His residue whose R-group must be deprotenated in order for the hormone to be active. The hormone is fully active if this His is deprotenated and completely inactive if it is protenated. At what pH would you expect the hormone to have 67% of its maximal activity?
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Old 09-20-2012, 08:59 AM
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Thumbs up Re: You are characterizing a newly isolated peptide which serves as a hormone.?

By using Henderson-Hasselbalch Equation (pKa Value for imidazole used in calculation is 6)

pH 6.3, His:His+ = 2:1
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