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A small peptide was isolated and sequenced.What is the sequence if:?

A small peptide was isolated and sequenced.What is the sequence if:? - Peptide Forum

A small peptide was isolated and sequenced.What is the sequence if:? - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


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Old 02-13-2012, 01:16 AM
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Default A small peptide was isolated and sequenced.What is the sequence if:?



A small peptide was isolated and sequenced. What is the sequence if:
A. Total hydrolysis yielded L, F and Y in molar ratios and G in a ratio of two.
B. Treatment with Sanger reagent gave DNP-Tyr.
C. Pepsin which cuts the chain at the N-end of aromatic amino acids gave a
dipeptide and a tripeptide.
D. Each peptide (of procedure C) was hydrolyzed, the dipeptide yielded F and L
The tripeptide yielded Y and G in a 1:2 ratio.
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Old 02-13-2012, 05:57 AM
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Smile Re: A small peptide was isolated and sequenced.What is the sequence if:?

Answer is NH2-Y-G-G-F-L-COOH

Sanger reagent react with amino terminal so You get DNP-Tyr
Pepsin break the sequence at amino component of Phe(F) so you get two peptide 1) Y-G-G (1:2)
2) F-L

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Kunal pandya
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