I am looking for a signalling molecule in phloem sap extract. The signalling molecule can be carbohydrate, hormone or peptide in nature. The problem is that the extraction buffer used for phloem sap extraction has to contain EDTA and this disrupts the bio-assay that I am using to search for this signalling molecule, so it needs to be removed after exudation.
I have looked up methods for recovering EDTA (doi:10.1016/S0304-3894(03)00268-1) by acidifying the solution with concentrated H2SO4 (pH<3) which precipitates H4EDTA (removes about 90-95%). I have an issue because adding conc. H2SO4 can react with sugars and other compounds.
Does any body know a way of removing EDTA without resulting in unwanted reactions?
EDTA Conc. of exudation buffer = 20mM