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What is the net charge on this peptide at pH 7?

What is the net charge on this peptide at pH 7? - Peptide Forum

What is the net charge on this peptide at pH 7? - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


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Old 10-30-2011, 02:17 PM
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Default What is the net charge on this peptide at pH 7?



H-E-D-Q-R-M-M-C-L-K-L-P-E-D-V-A-A-D-K-K-A-P-Y

Also, why?
Sorry, the peptide is:

H-E-D-Q-R-M-M-C-L-K-L-P-E-D-V-A-A-D-K-K-A-P-Y


After the final K-K comes A-P-Y.

For some reason, it wouldnt let me c&p the whole thing together.
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Old 10-31-2011, 07:22 PM
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Default Re: What is the net charge on this peptide at pH 7?

Here is the answer for you. Net charge at pH7 is -1. Let me know if you need help on peptide synthesis.


Net charge at pH 7.0:-1
Iso-electric point, pI:5.4 Average hydrophilicity: 0.6
Ratio hydrophilic residues / total number of residues: 43%

Interpreted sequence
1-letter code: HEDQRMMCLKLPEDVAADKKAPY
3-letter code: His-Glu-Asp-Gln-Arg-Met-Met-Cys-Leu-Lys-Leu-Pro-Glu-Asp-Val-Ala-Ala-Asp-Lys-Lys-Ala-Pro-Tyr

Number of residues:
23
Molecular weight, MW:
2689.1
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