GFP is fused to a signal peptide, and no other sequence, and transfected into a cell. Where is the exact fluorescence when the chimeric protein has reached its final destination, and the cell is examined by fluorescence microscopy?
a) in the lysosome lumen
b) in the ER lumen
c) on the plasma membrane on the cytosolic side
d) on the plasma membrane on the extracellular side
e) in the extracellular space/ ECM
2. Where is the exact fluorescence when a stop transfer sequence is added at the most carboxy terminus to the protein above (Question 1)