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If the enzyme is Pepsin and the end product is Peptides what is the substrate?

If the enzyme is Pepsin and the end product is Peptides what is the substrate? - Peptide Forum

If the enzyme is Pepsin and the end product is Peptides what is the substrate? - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


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Old 09-25-2011, 03:16 PM
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Default If the enzyme is Pepsin and the end product is Peptides what is the substrate?



Hope somebody can help
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Old 09-25-2011, 03:16 PM
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Proteins
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Old 09-25-2011, 06:06 PM
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Default Re: If the enzyme is Pepsin and the end product is Peptides what is the substrate?

Pepsin, hydrolyzes only peptide bonds present in Protein(Substrate). It does not hydrolyze non-peptide amide or ester linkages.

Pepsin exhibits preferential cleavage for hydrophobic,aromatic residues . Increased susceptibility to hydrolysis occurs if there is a sulfur-containing amino acid close to the peptide bond, which has an aromatic amino acid.
Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the B chain of insulin.

Pepsin will also preferentially cleave at the carboxyl side of phenylalanine and leucine and to a lesser extent at the carboxyl side of glutamic acid residues. Pepsin will not cleave at valine, alanine, or glycine linkages.Amidation of the C-terminal carboxyl group prevents hydrolysis by pepsin.
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