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How to calculate the yield...

How to calculate the yield... - Peptide Forum

How to calculate the yield... - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


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Old 05-17-2011, 01:02 PM
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Default How to calculate the yield...



Hey guys,

I have troubles to calculate the yield of a peptide synthesis. The point is that I dont understand how I have to use the substitution of the aminoacid in the resin. I will put you one example:

Peptide that I want to synthesize: Lys-Ser-Ala-Wang Resin
Fmoc-Ala-Wang Resin (substitution 0.72 mmol/g)

the goal is to synthesize 0.1 mmol peptide so it means that I have to use 0.139 g of the a.a.-resin (m=0.1mmol peptide/0.72 mmol/g = 0.139g).

So how I have to calculate the theoretical yield?

Thanks for advance
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Old 06-06-2011, 10:08 AM
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Thumbs up Re: How to calculate the yield...

Their is two way of calculation.

1) Fmoc-Value of first amino acid (i.e. Fmoc-Ala-Wang Resin) is your theoretical 100% yield . If you get fmoc-Value for Fmoc-Ala-WR is 0.1mm/gm and final Qty of tri peptide is 0.05mm/gm then your yield is 50%

2) Fmoc-Value of last amino acid (i.e. Fmoc-Lys-2PP-WR) is taken as 100% yield (Theoretical Yield) and on basis of that calculate Final yield

Regards
Kunal Pandya
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