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Peptide Sequence Sysnthesis - Reproducibility?
I make the same peptide sequence multiple times moving the placement of a Cys every synthesis. I notice that my peptides lately have been giving really poor results. The HPLC gives one single peak which is about 8-10 aa long. Previously I was only seeing one peak also but it was full length (23aa).
Let me know if anyone has any suggestions?
My resin and reagents are the same. I decreased the activator (HBTU) to about .45M and it helped a bit.
Last edited by admin; 05-03-2011 at 02:20 AM.
|peptide , reproducibility , sequence , sysnthesis|
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