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Peptide Sequence Sysnthesis - Reproducibility?

Peptide Sequence Sysnthesis - Reproducibility? - Peptide Forum

Peptide Sequence Sysnthesis - Reproducibility? - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


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Old 05-03-2011, 12:06 AM
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Question Peptide Sequence Sysnthesis - Reproducibility?



Hi folks,
I make the same peptide sequence multiple times moving the placement of a Cys every synthesis. I notice that my peptides lately have been giving really poor results. The HPLC gives one single peak which is about 8-10 aa long. Previously I was only seeing one peak also but it was full length (23aa).
Let me know if anyone has any suggestions?
My resin and reagents are the same. I decreased the activator (HBTU) to about .45M and it helped a bit.
CHeers,
Borr

Last edited by admin; 05-03-2011 at 02:20 AM.
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