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-   -   Peptide Sequence Sysnthesis - Reproducibility? (http://www.molecularstation.com/forum/peptide-forum/82522-peptide-sequence-sysnthesis-reproducibility.html)

Borr 05-03-2011 12:06 AM

Peptide Sequence Sysnthesis - Reproducibility?
 
Hi folks,
I make the same peptide sequence multiple times moving the placement of a Cys every synthesis. I notice that my peptides lately have been giving really poor results. The HPLC gives one single peak which is about 8-10 aa long. Previously I was only seeing one peak also but it was full length (23aa).
Let me know if anyone has any suggestions?
My resin and reagents are the same. I decreased the activator (HBTU) to about .45M and it helped a bit.
CHeers,
Borr


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