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how to calculate

how to calculate - Peptide Forum

how to calculate - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


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Old 02-23-2011, 06:16 PM
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Hello,dose anybody knows how to preparate 1,5 gram resin with 0,2 mmol/g substitution using 0,8 mmol/g resin,what scale?
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Old 02-26-2011, 09:45 PM
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Default Re: how to calculate

Hi
Both the symmetrical anhydride method (10 eq aa to generate 5 eq anhydride) and the MSNT/MeIm method uses 5 eq amino acid (aa) with respect to the resin loading. So you might wanna start out with 1.25 eq aa, and then take out a small sample and dertermine the loading, if the loading is within the expected range cap with benzoic anhydride, if not repeat the procedure with fewer eq aa.

\Erik
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