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Determine the amino acid sequence of a peptide using the following information:?

Determine the amino acid sequence of a peptide using the following information:? - Peptide Forum

Determine the amino acid sequence of a peptide using the following information:? - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


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Old 09-08-2009, 11:16 AM
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Default Determine the amino acid sequence of a peptide using the following information:?



a.A nonapeptide was determined to have the following amino acid composition: (Lys)2, (Gly) 2, (Phe) 2, His, Leu, Met.

b.The native peptide was incubated with 1-fluoro-2,4-dinitrobenzene (FDNB) and then hydrolyzed; 2,4-dinitrophenylhistidine was identified.

c.When the native peptide was exposed to cyanogen bromide (CNBr), an octapeptide and free glycine were recovered.

d.Incubation of the native peptide with trypsin gave a pentapeptide, a tripeptide, and free Lys. 2,4-Dinitrophenyl-histidine was recovered from the pentapeptide, and 2,4-dinitrophenylphenylalanine was recovered from the tripeptide.

e.Digestion with the enzyme pepsin produced a dipeptide, a tripeptide, and a tetrapeptide. The tetrapeptide was composed of (Lys) 2, Phe, and Gly.
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