what is a spacer sequence in a peptide?

[ken]

what is the role of a spacer sequence in a peptide?

[musgun]

I think the name says it all When you make a fusion peptide or protein, you may want to spatially separate the two components. The sequence you put in between is the linker or the spacer sequence. People usually use multiple repeats of flexible amino acids such as G,S, rigid amino acids such as P. or combinations of these such as GPGPGP. You can also use alpha helix motives as spacers if you need more separation.

[glbiochem]

There are many different spacers provide for you.
{Mini-PEG}
{Ava}, C5
{Ahx}, C6
{GABA}, C4
{Beta-Ala}, C3
{Gly}, C2

It can help you link the fluoro groups with peptide or two antigen parts together.
From our experience, C6 and {Mini-PEG} are good in length.
C2 and C3 are too short, their structure is reported instable.

[marisavarmor]

Hi, searching on the web I find your answer. However, I was looking for references about optimal spacers.

Please, could you help me?

We have synthesized a peptide 15-mer with an additional 6x-Histag in C-terminal end. We would like to try a spacer sequence between them.

Thanks.

[glbiochem]

There is no reference. Just our experience.
In your case, I feel {Ahx} is suitable.