Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Proteomics Forum > Peptide Forum
Register Search Today's Posts Mark Forums Read

Peptide Forum Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


Methods for storing uncompleted peptide synthesis

Methods for storing uncompleted peptide synthesis - Peptide Forum

Methods for storing uncompleted peptide synthesis - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-31-2009, 10:52 PM
Pipette Filler
Points: 292, Level: 5 Points: 292, Level: 5 Points: 292, Level: 5
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2009
Posts: 6
Thanks: 0
Thanked 0 Times in 0 Posts
Default Methods for storing uncompleted peptide synthesis



Hey Guys, i ve had a problem with synthesizing long peptides[15-20 mers] as they cannot be synthesized in a day and needs to be kept over night before i can start again with the synthesis. Any suggestion as to how can i store the uncomplete peptide overnight?
what i used to do was dry the peptide in vacuum, but this may cause collapsing of the peptide,
is it a good idea to leave the peptide in minimal DCM overnight?
Reply With Quote
  #2  
Old 06-01-2009, 03:28 AM
Pipette Filler
Points: 986, Level: 17 Points: 986, Level: 17 Points: 986, Level: 17
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2008
Posts: 16
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Methods for storing uncompleted peptide synthesis

I think you need not dry the resin. Just leave them there and start coupling the next day
Reply With Quote
  #3  
Old 06-02-2009, 05:40 AM
Pipette Filler
Points: 629, Level: 12 Points: 629, Level: 12 Points: 629, Level: 12
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2008
Posts: 4
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Methods for storing uncompleted peptide synthesis

Hej hej,
you can let it in solution over night.
Good luck
Reply With Quote
  #4  
Old 06-03-2009, 05:09 AM
Pipette Filler
Points: 292, Level: 5 Points: 292, Level: 5 Points: 292, Level: 5
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2009
Posts: 6
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Methods for storing uncompleted peptide synthesis

Thanks all. i will take them in consideration while synthesizing my next peptide
Reply With Quote
  #5  
Old 06-29-2009, 07:28 PM
Pipette Filler
Points: 2,314, Level: 31 Points: 2,314, Level: 31 Points: 2,314, Level: 31
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2007
Posts: 25
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: Methods for storing uncompleted peptide synthesis

As long as it is in DMF or DCM it will be stable. But if an automated synthesis stops at the activation or the deprotection step, this may cause problems.
Reply With Quote
  #6  
Old 08-27-2009, 11:18 AM
Pipette Filler
Points: 1,115, Level: 19 Points: 1,115, Level: 19 Points: 1,115, Level: 19
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2008
Location: Amsterdam
Posts: 10
Thanks: 0
Thanked 2 Times in 2 Posts
Default Re: Methods for storing uncompleted peptide synthesis

Stop the synthesis before the deprotection step. You can dry NMP of DMF whatever you use. Put the resin in the fridge at 4 C. The next day wash the resin with NMP or DMF en then start the synthesis.
Reply With Quote
Reply

Tags
methods , peptide , storing , synthesis , uncompleted


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
disulfide cleavage dpar Peptide Forum 5 08-20-2010 01:09 PM
What are the different techniques are there in peptide synthesis such N- Kaushil Peptide Forum 1 09-01-2009 12:33 PM
Custom Peptide Synthesis Company sinopep03 Peptide Forum 0 01-04-2009 02:15 AM
96 well plate peptide synthesis service glbiochem Chit Chat 0 11-15-2007 05:22 AM
Custom Gene Synthesis or Peptide Synthesis biomatik Molecular Biology Products and Vendors 0 10-05-2007 03:54 PM


All times are GMT. The time now is 02:56 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14234 seconds with 16 queries