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what rapid coupling protocols do you use?

what rapid coupling protocols do you use? - Peptide Forum

what rapid coupling protocols do you use? - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


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  #1  
Old 04-27-2009, 06:09 AM
Pipette Filler
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Default what rapid coupling protocols do you use?



hi proficients!
I want to know some rapid coupling protocols for peptide synthesis,could anybody give me a suggestion?
Now i am using HBTU,but i find it isn`t as extremely efficient as my expectations.
How long it takes when using HBTU/DIEA in the ordinary coupling?
What methods do you use for difficult peptide synthesis?

Thanks a lot!
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Old 04-27-2009, 04:31 PM
Pipette Filler
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Default Re: what rapid coupling protocols do you use?

We've found that HCTU is a highly efficient activator - similar in efficiency to HATU, but at a much lower cost. We have some posters and references to papers we published on the topic in the Journal of Peptide Science on our website. Just Google Protein Technologies Inc, then click on Applications. We're a peptide synthesizer manufacturer, but our chemistry team has been working on fast chemistry for the last few years.

When we use HBTU, typically we start with 2x30 min coupling times for ordinary couplings, but we've been able to go as low as 2x10 min depending on the sequence.

With HCTU, we've been able to go as low as 2x1 min coupling times depending on the sequence, so you can see the difference!

Ideas for difficult sequences include using a low-loaded resin (~0.2 mmol/g instead of ~0.5 mmol/g). This helps for hydrophobic or long sequences. Also, try using DMSO as the solvent in the coupling reaction, or a 1:1 DMF/DMSO mixture. We've found this to help as well as long as there is not a methionine in the sequence because it will get oxidized and have to be reduced at the end of the synthesis.

Hope this helps! If you want to contact us directly with your questions, you can contact us from our website as well.
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The Following User Says Thank You to Bluwatch For This Useful Post:
long67865 (04-28-2009)
  #3  
Old 04-28-2009, 01:22 AM
Pipette Filler
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Default Re: what rapid coupling protocols do you use?

hi Bluwatch!
thanks a lot for your help!
i have logged onto your website ,it's really nice! But it's a pity that i don`t know how can i get in touch with you.
i don't quite understand that do you double couple all the amino acids?

Last edited by long67865; 04-28-2009 at 04:48 AM.
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Old 04-29-2009, 06:53 AM
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Default Re: what rapid coupling protocols do you use?

HATU is much faster than HBTU, for difficult peptide synthesis we use HATU and DIC alternatively.
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Old 04-30-2009, 03:39 PM
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Default Re: what rapid coupling protocols do you use?

Hi Long,

That was my first post, and I can't post an email address or website until I've posted 5 times, but you can go to our Contact us page on our website and just say you'd like to speak with Christina in the comments area, and it will be forwarded to me. I'll be happy to discuss anything with you! Hope to hear from you soon!
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