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peptide synthesis of MCOT-II cyclotide

peptide synthesis of MCOT-II cyclotide - Peptide Forum

peptide synthesis of MCOT-II cyclotide - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


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  #1  
Old 01-22-2009, 02:25 PM
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Default peptide synthesis of MCOT-II cyclotide



Any ideas for synthesis of MCOT-II cyclotide synthesis chemically????
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  #2  
Old 01-28-2009, 05:51 AM
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Default Re: peptide synthesis of MCOT-II cyclotide

sequence?
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  #3  
Old 01-29-2009, 03:47 PM
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Default Re: peptide synthesis of MCOT-II cyclotide

CPKILKKCRRDSDCPGACICRGNGYCGSGSDGG[V]

thanks
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  #4  
Old 02-02-2009, 04:01 AM
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Default Re: peptide synthesis of MCOT-II cyclotide

cyclo{Cys-Pro-Lys-Ile-Leu-Lys-Lys-Cys-Arg-Arg-Asp-Ser-Asp-Cys-Pro-Gly-Ala-Cys-Ile-Cys-Arg-Gly-Asn-Gly-Tyr-Cys-Gly-Ser-Gly-Ser-Asp-Gly-Gly-Val} .right?
mybe we can make a run ,try to synthesis
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  #5  
Old 02-02-2009, 07:10 AM
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Default Re: peptide synthesis of MCOT-II cyclotide

thts fine.....the prob is which sequence to use so that after the synthesis the linear peptide can be cyclised. a cyclotide lke mcot-II can be divided in 6 different sequences[using the above mentioned residues]..the main issue is selection of the 6 sequences in an order so it cyclises...usually if proper selection of sequences and ends is not done..neither it wil form the three cysteine loops..nor wil it cyclise
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Old 02-03-2009, 11:34 AM
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Default Re: peptide synthesis of MCOT-II cyclotide

let's talk about the details , design the synthesis scheme? and so on,,,,,,

Last edited by admin; 02-03-2009 at 08:54 PM. Reason: please no emails
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  #7  
Old 05-01-2009, 11:09 AM
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Default Re: peptide synthesis of MCOT-II cyclotide

You can use three different kinds of protected group and use DMSO to cyclise the Cys you want. We have the experience to synthesize to two couples of Cys, but three couples of Cys is still in experiment.
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Old 05-02-2009, 05:54 AM
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Default Re: peptide synthesis of MCOT-II cyclotide

yes that seems reasonable
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cyclotide , mcotii , peptide , synthesis


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