| | Re: synthetic peptide purification , help
It seems that the peptide's N terminus is some aggrigation.
I usually use "peptide companion" to predict it. However, I'm a sales person, not technician. The predict result is not always consistent with the real result.
I just use this software result to provide some suggestion for my customer.
Regarding to your sequence, maybe you can consider below 3 points:
Whether each cycle coupling has been fully completed, especially at the N terminus?
Whether the peptide needs to full reduction with EDT?
Whether have you dissolved it well before HPLC preparation?