| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Peptide Forum Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
12-22-2008, 04:38 PM
| |||||||||||
| |||||||||||
 synthetic peptide purification , help  hi my peptide has 18 aa with 50% hydrophobicity, i do't know why my c18 RP- column can not purify it. all peaks are ligated to each other . what i have to do?  |
|
#3
12-23-2008, 05:33 AM
| |||||||||||
| |||||||||||
 Re: synthetic peptide purification , help hello how i can recognize that it has been aggrigated or not? the sequence: GLAANCKRTIKCFLPKIF thanks for your attention |
|
#4
12-25-2008, 04:18 AM
| |||||||||||
| |||||||||||
| Re: synthetic peptide purification , help It seems that the peptide's N terminus is some aggrigation. I usually use "peptide companion" to predict it. However, I'm a sales person, not technician. The predict result is not always consistent with the real result. I just use this software result to provide some suggestion for my customer. Regarding to your sequence, maybe you can consider below 3 points: Whether each cycle coupling has been fully completed, especially at the N terminus? Whether the peptide needs to full reduction with EDT? Whether have you dissolved it well before HPLC preparation? |
|
#5
12-25-2008, 06:12 AM
| |||||||||||
| |||||||||||
| Re: synthetic peptide purification , help Dear glbiochem really thanks for your attention and help |
|
#6
12-31-2008, 02:02 PM
| |||||||||||
| |||||||||||
| Re: synthetic peptide purification , help you can try to use new HPLC column, or add some acetic acid or TFA to the sample assite dissovled. Last edited by zjuer; 12-31-2008 at 03:52 PM. Reason: company |
|
| Tags |
| peptide , purification , synthetic |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| disulfide cleavage | dpar | Peptide Forum | 5 | 08-20-2010 01:09 PM |
| Plant Synthetic Biology Post Doctoral Positions | Medford,June | Arabidopsis and Plant Biology | 0 | 02-04-2009 10:18 PM |
| Peptide Aldehyde Synthesis and Purification | puri | Peptide Forum | 4 | 04-18-2008 12:12 AM |
| beta sheet peptide purification | jthundim | Peptide Forum | 2 | 11-16-2007 01:18 AM |
| Small peptide purification blues | samreen shaikh | Protein Forum | 1 | 07-25-2007 08:21 PM |
Linear Mode
