protocol for solution phase peptide synthesis

[peptideraja]

Dear Frnds,
Can anyone tell me the manual way of Deprotection, Work-up and coupling,
In Solution Phase Peptide Synthesis? Thank you

[zjuer]

hi
Can you say more details ,

[peptideraja]

Hi Boss,

Thanks for your interest on my query.

Target Peptide is Boc-Leu-Val-Ile-OMe

i will couple Boc-Leu-Val-OH + H2N-Ile-Ala-OMe.

My problem is, After De-Boc'ing of Boc-Ile-Ala-OMe with Formic Acid,i can remove the formic acid after that how to work-up should takes place with care,those procedure if you provide i will be grateful.becoz sometime i am not getting any yield.

Thank you so much

[glbiochem]

Anybody who need to make peptide C terminus modification, may contact us for below reagents
H-Ala-NHME.HCL
H-Arg-NHME.2HCL
H-Asn-NHME.HCL
H-Asp(OtBu)-NHME.HCL
H-Asp(OBzl)-NHME.HCL
H-Cys(Acm)-NHME.HCL
H-Cys(Trt)-NHME.HCL
H-Glu(OBzl)-NHME.HCL
H-Glu(OtBu)-NHME.HCL
H-Gln(Xan)-NHME.HCL
H-Gln-NHME.HCL
H-Gln(Trt)-NHME.HCL
H-Gly-NHME.HCL
H-His(Bzl)-NHME.HCL
H-His(Trt)-NHME.HCL
H-Ile-NHME.HCL
H-Leu-NHME.HCL
H-Lys(Z)-NHME.HCL
H-Lys(Boc)-NHME.HCL
H-Met-NHME.HCL
H-Phe-NHME.HCL
H-Pro-NHME.HCL
H-Ser(Bzl)-NHME.HCL
H-Ser(tBu)-NHME.HCL
H-Thr(Bzl)-NHME.HCL
H-Thr(tBu)-NHME.HCL
H-Trp(For)-NHME.HCL
H-Trp-NHME.HCL
H-Trp(Boc)-NHME.HCL
H-Tyr(Bzl)-NHME.HCL
H-Tyr(tBu)-NHME.HCL
H-Val-NHME.HCL
H-Ala-NHME2.HCL
H-Arg-NHME2.2HCL
H-Asn-NHME2.HCL
H-Asp(OtBu)-NHME2.HCL
H-Asp(OBzl)-NHME2.HCL
H-Cys(Acm)-NHME2.HCL
H-Cys(Trt)-NHME2.HCL
H-Glu(OBzl)-NHME2.HCL
H-Glu(OtBu)-NHME2.HCL
H-Gln(Xan)-NHME2.HCL
H-Gln-NHME2.HCL
H-Gln(Trt)-NHME2.HCL
H-Gly-NHME2.HCL
H-His(Bzl)-NHME2.HCL
H-His(Trt)-NHME2.HCL
H-Ile-NHME2.HCL
H-Leu-NHME2.HCL
H-Lys(Z)-NHME2.HCL
H-Lys(Boc)-NHME2.HCL
H-Met-NHME2.HCL
H-Phe-NHME2.HCL
H-Pro-NHME2.HCL
H-Ser(Bzl)-NHME2.HCL
H-Ser(tBu)-NHME2.HCL
H-Thr(Bzl)-NHME2.HCL
H-Thr(tBu)-NHME2.HCL
H-Trp(For)-NHME2.HCL
H-Trp-NHME2.HCL
H-Trp(Boc)-NHME2.HCL
H-Tyr(Bzl)-NHME2.HCL
H-Tyr(tBu)-NHME2.HCL
H-Val-NHME2.HCL
H-Ala-AMC.HCL
H-Arg-AMC.2HCL
H-Asn-AMC.HCL
H-Asp(OtBu)-AMC.HCL
H-Asp(OBzl)-AMC.HCL
H-Cys(Acm)-AMC.HCL
H-Cys(Trt)-AMC.HCL
H-Glu(OBzl)-AMC.HCL
H-Glu(OtBu)-AMC.HCL
H-Gln(Xan)-AMC.HCL
H-Gln-AMC.HCL
H-Gln(Trt)-AMC.HCL
H-Gly-AMC.HCL
H-His(Bzl)-AMC.HCL
H-His(Trt)-AMC.HCL
H-Ile-AMC.HCL
H-Leu-AMC.HCL
H-Lys(Z)-AMC.HCL
H-Lys(Boc)-AMC.HCL
H-Met-AMC.HCL
H-Phe-AMC.HCL
H-Pro-AMC.HCL
H-Ser(Bzl)-AMC.HCL
H-Ser(tBu)-AMC.HCL
H-Thr(Bzl)-AMC.HCL
H-Thr(tBu)-AMC.HCL
H-Trp(For)-AMC.HCL
H-Trp-AMC.HCL
H-Trp(Boc)-AMC.HCL
H-Tyr(Bzl)-AMC.HCL
H-Tyr(tBu)-AMC.HCL
H-Val-AMC.HCL
H-Ala-PNA.HCL
H-Arg-PNA.2HCL
H-Asn-PNA.HCL
H-Asp(OtBu)-PNA.HCL
H-Asp(OBzl)-PNA.HCL
H-Cys(Acm)-PNA.HCL
H-Cys(Trt)-PNA.HCL
H-Glu(OBzl)-PNA.HCL
H-Glu(OtBu)-PNA.HCL
H-Gln(Xan)-PNA.HCL
H-Gln-PNA.HCL
H-Gln(Trt)-PNA.HCL
H-Gly-PNA.HCL
H-His(Bzl)-PNA.HCL
H-His(Trt)-PNA.HCL
H-Ile-PNA.HCL
H-Leu-PNA.HCL
H-Lys(Z)-PNA.HCL
H-Lys(Boc)-PNA.HCL
H-Met-PNA.HCL
H-Phe-PNA.HCL
H-Pro-PNA.HCL
H-Ser(Bzl)-PNA.HCL
H-Ser(tBu)-PNA.HCL
H-Thr(Bzl)-PNA.HCL
H-Thr(tBu)-PNA.HCL
H-Trp(For)-PNA.HCL
H-Trp-PNA.HCL
H-Trp(Boc)-PNA.HCL
H-Tyr(Bzl)-PNA.HCL
H-Tyr(tBu)-PNA.HCL
H-Val-PNA.HCL
H-Ala-NHME2.HCL
H-Arg-NHME2.2HCL
H-Asn-NHME2.HCL
H-Asp(OtBu)-NHME2.HCL
H-Asp(OBzl)-NHME2.HCL
H-Cys(Acm)-NHME2.HCL
H-Cys(Trt)-NHME2.HCL
H-Glu(OBzl)-NHME2.HCL
H-Glu(OtBu)-NHME2.HCL
H-Gln(Xan)-NHME2.HCL
H-Gln-NHME2.HCL
H-Gln(Trt)-NHME2.HCL
H-Gly-NHME2.HCL
H-His(Bzl)-NHME2.HCL
H-His(Trt)-NHME2.HCL
H-Ile-NHME2.HCL
H-Leu-NHME2.HCL
H-Lys(Z)-NHME2.HCL
H-Lys(Boc)-NHME2.HCL
H-Met-NHME2.HCL
H-Phe-NHME2.HCL
H-Pro-NHME2.HCL
H-Ser(Bzl)-NHME2.HCL
H-Ser(tBu)-NHME2.HCL
H-Thr(Bzl)-NHME2.HCL
H-Thr(tBu)-NHME2.HCL
H-Trp(For)-NHME2.HCL
H-Trp-NHME2.HCL
H-Trp(Boc)-NHME2.HCL
H-Tyr(Bzl)-NHME2.HCL
H-Tyr(tBu)-NHME2.HCL
H-Val-NHME2.HCL

[zjuer]

i see ,the procedure may be the problem .you can try to use 50%TFA/DCM 1h DeBoc,then evaporate the solution to dryness, then wash the precipitation with 1M sodium carbonate/water solution ,collect the precipitates by filter ,dry the product in desiccator. 6M hydrochloric(gas)/dioxane solution also works well.
Formic Acid can react with amimes after you deBOC.it's a side rection .
i hope the procedure can help you

[peptideraja]

Hi Brother,
Thanks a lot for your kind reply.
I will try your method and post you the reply.
Thanks a lot

[hadjer]

i need to know what is the best way to extract a protines from a mucilagineus tissus from a plant