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need help understanding what n and c terminus on hHGRH(1-29)

need help understanding what n and c terminus on hHGRH(1-29) - Peptide Forum

need help understanding what n and c terminus on hHGRH(1-29) - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


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Old 11-16-2008, 12:29 AM
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Default need help understanding what n and c terminus on hHGRH(1-29)



What is the n-terminus? is the H- for Histidine on the front of the peptide below stand for the N-terminus, and because its an amino acid make it an amine terminus. If the C-terminus is NH2 is that thin an amide on the N-terminus? or amine.


H-Tyr-(D) Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH2

With the hGHRH(1−29)-NH2 sequence and there are 4 substitutions on positions 2, 8, 15 and 27. Is this what gives it its longer halflife of 7 days? and thats why they call it cjc-1295
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Old 11-19-2008, 09:58 PM
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Default Re: need help understanding what n and c terminus on hHGRH(1-29)

Quote:
Originally Posted by will-Jr View Post
What is the n-terminus? is the H- for Histidine on the front of the peptide below stand for the N-terminus, and because its an amino acid make it an amine terminus. If the C-terminus is NH2 is that thin an amide on the N-terminus? or amine.


H-Tyr-(D) Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH2

With the hGHRH(1−29)-NH2 sequence and there are 4 substitutions on positions 2, 8, 15 and 27. Is this what gives it its longer halflife of 7 days? and thats why they call it cjc-1295
Convention for sequence writing peptides is Left (N-terminus) to Right (C-terminus). The left terminus "H-" refers to the explicit nature of the amino group being "free" and NOT acetylated, but could still be a salt form like a hydrochloride salt. The C-terminus "-NH2" refers to the fact it is NOT a free carboxylic acid but rather a primary amide. A term like "-NH(CH3)" or "-NHMe" implies "methyl-amide" c-terminus on the peptide.
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Old 11-24-2008, 01:46 AM
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Default Re: need help understanding what n and c terminus on hHGRH(1-29)

H- means amino group at the N terminus, -NH2 means the amidation at the C terminus not COOH(free acid).

This is the plain sequence of CJC-1295, the real CJC-1295 has a modification at the C terminus with Lys(Mal). But the modified CJC-1295 is very hard to make and costly. The most product sold is the plain one.
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Old 12-31-2008, 03:27 PM
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Default Re: need help understanding what n and c terminus on hHGRH(1-29)

H- means amino group at the N terminus, -NH2 means the amidation at the C terminus not COOH(free acid).
This is the plain sequence of CJC-1295, the real CJC-1295 has a modification at the C terminus with Lys(Mal). the modified CJC-1295 is not very hard to make , but he most product sold is the plain one.
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