Hi, I'm trying to isolate EDT effectively without stenching up my whole lab with toxic fumes. I've been using an ether precipitation/centrifugation method. This is quite tedious, as it entails multiple ether precipitation/centrifugations and my centrifuge isn't in the hood which isn't good and I'd rather choose another method. I guess I could try to filter my solution, but it seems I lose a lot of my peptide due to the filtering. I'm thinking of getting a roto-vap in the hood, any good pros and cons to this? Or would getting a spark-free centrifuge be sufficient, inside the hood?
Also, I have a peptide in aqueous PBS buffer now that still has EDT contamination. Any tips on how to get rid of the EDT besides lyophilization? I tried a liquid-liquid extraction 1:1 with Ether but I didn't see any layers at all.
Thanks in advance!