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How to check peptide aggregation in solution?

How to check peptide aggregation in solution? - Peptide Forum

How to check peptide aggregation in solution? - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


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Old 09-09-2008, 05:01 PM
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Default How to check peptide aggregation in solution?



Hello folks,

I would like to know whether my peptide exists as a monomer or dimer, or aggregates in aqueous solution. Can anyone suggest reliable ways to confirm it? Many thanks in advance.
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Old 09-12-2008, 10:24 AM
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Default Re: How to check peptide aggregation in solution?

Do an HPLC and confirm it with maldi or esi. If the maldi gives you a single peak but your hplc shows you 2 or more peaks, that means your peptide is aggregating. Sequence of u r peptides also helps us to predict aggregation.
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