capping

[a_m_shaheen]

i am trying make 20 aa peptide. it is my first time to do so. i am doing it automatically and i wonder if anybody add a capping step to the synthesis program inorder to enhance the quality of the formed peptide

[ask03]

usually the longer the peptide,the more necessary it becomes to cap tp prevent side interactions and aggregation

[musgun]

as usual, depends on the sequence. 20 a.a. s doesn't sound too long and if you have some prolines in your sequence you usually can get away without using capping agent.

[a_m_shaheen]

Originally Posted by ask03

usually the longer the peptide,the more necessary it becomes to cap tp prevent side interactions and aggregation

thanks

[a_m_shaheen]

Originally Posted by musgun

as usual, depends on the sequence. 20 a.a. s doesn't sound too long and if you have some prolines in your sequence you usually can get away without using capping agent.

thanks

[Lysin]

Hej hej,
I would say, it is necessary to cap. If you want to have only one sequence and a good yield. If you need some information use the novabiochem catalog, it explains a lot. You also ca order from Bachem a small SPPS guidline for free.
Good luck

[ask03]

Originally Posted by Lysin

Hej hej,
I would say, it is necessary to cap. If you want to have only one sequence and a good yield. If you need some information use the novabiochem catalog, it explains a lot. You also ca order from Bachem a small SPPS guidline for free.
Good luck

how do you order a SPPS guideline for free? does it have to accompany some other order??

[a_m_shaheen]

i filled the order requist online but no body reply me