Multiple Antigenic Peptide systems

[ask03]

Has anyone in this forum worked with or read about the multimeric peptide system using MAPS?? If yes please reply. Thanks

[glbiochem]

We made them before. It was several same seqeunces linked through Lys two amine groups. We made them by coupling amino acids one by one on MAPS beads, Fmoc8-Lys4-Lys2-Lys-Resin or Fmoc4-Lys2-Lys-Resin

But we can not make purification on such kind of sequence. Its purity is really too bad.

There is another synthesis method. Make one full protect sequence on 2-CTC resin, cleave from resin, then couple it to MAPS beads.
The second method has difficulty to make each link sites were fully conjugated.
But it can promise the linked sequence were correct.

I'm not sure which method is prefered. However, both can not get pure sample by HPLC.

If you do not so many branches on MAPS, for example 2 branches, we can make pure sample and unique M.W.

[ask03]

HI glbiochem. Thanks for your info. I had some questions. Why do you think the MAPS cant be purified? Did you refer to any of the papers which have done purification on the MAPS??

[ask03]

Originally Posted by glbiochem

We made them before. It was several same seqeunces linked through Lys two amine groups. We made them by coupling amino acids one by one on MAPS beads, Fmoc8-Lys4-Lys2-Lys-Resin or Fmoc4-Lys2-Lys-Resin

But we can not make purification on such kind of sequence. Its purity is really too bad.

There is another synthesis method. Make one full protect sequence on 2-CTC resin, cleave from resin, then couple it to MAPS beads.
The second method has difficulty to make each link sites were fully conjugated.
But it can promise the linked sequence were correct.

I'm not sure which method is prefered. However, both can not get pure sample by HPLC.

If you do not so many branches on MAPS, for example 2 branches, we can make pure sample and unique M.W.

hi glbiochem

u said u had made MAPS before right. can u please tell me the following things...
1.what was the loading capacity of the resin you used?
2.what was the sequence of the peptide you coupled onto the Lys core?
3. what solvents did you use? what deprotecting and cleavage reagent?
4. what activating agent?
5. how much excess of amino acid?
6. what was the coupling time for each AA.

Actually I am just unable to make it. Coupling is not successful at all. Will be grateful if you could provide these details to me.

Thanks,

Ask03

[zjuer]

we successed synthesis four branch MAP peptide in both solid phase and solution phase

the final product purity is over 98%

if you interested in you can email to me