Originally Posted by peppy
Microwave-assisted SPPS is proven to increase efficiency of coupling and deprotection (thus higher yields) in difficult sequences. For a good recent paper see International Journal of Peptide Research and Therapeutics, Vol. 13, Nos. 1–2, June 2007 pp. 203–208.
Thx for your reply and suggestion~
I have one more question on the topic, how about using the mixed-solvent system such as DMSO/DMF (v:v=1:1 or other proportions) which can serve as the electron donor solvent?
Or addition of chaotropic salts such as Lithium chloride to the reaction solvent?
As for microwave-assisted SPPS, specific apparatus should be used in order to confirm the reproducibility of SPPS. Microwave oven will not safe and cannot reach good reproducibility for our synthesis.