Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Proteomics Forum > Peptide Forum
Register Search Today's Posts Mark Forums Read

Peptide Forum Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


cyclic peptide

cyclic peptide - Peptide Forum

cyclic peptide - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-25-2008, 01:18 AM
Pipette Filler
Points: 1,248, Level: 20 Points: 1,248, Level: 20 Points: 1,248, Level: 20
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2007
Posts: 4
Thanks: 0
Thanked 0 Times in 0 Posts
Default cyclic peptide



I would like to do cyclic peptide using cysteine amino acids. Let me know more details, if any one expert in cyclic peptide chemistry in this forum.
thanks.
Reply With Quote
  #2  
Old 02-02-2008, 02:09 AM
Volunteer
Points: 2,271, Level: 30 Points: 2,271, Level: 30 Points: 2,271, Level: 30
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2007
Location: Shanghai
Posts: 66
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: cyclic peptide

How many disulfide bond in your sequence?
If only one, it would be quite easy.
You can use Fmoc-Cys(Trt)-OH insert in synthesis, and oxidate in water solution.

If over one, you need to choose Fmoc-Cys(Acm)-OH and Fmoc-Cys(tBu)-OH to oxidate and deprotect by different strategy.

Mr. David Wei
Mobile: 0086-15301650007(24 hour)
Tel: 0086-21-61263307
Fax: 0086-21-61263399
E-mail: [Only registered users see links. ]
Reply With Quote
  #3  
Old 02-25-2008, 03:26 AM
Pipette Filler
Points: 734, Level: 14 Points: 734, Level: 14 Points: 734, Level: 14
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2008
Location: Hyderabad
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: cyclic peptide

Cys(Acm) is good for peptide cyclisation, After syntheis of peptide, Deprotect the the peptide with cocktail and cyclised with Iodine in the presence of acid or alcohol.
Reply With Quote
  #4  
Old 04-18-2008, 12:27 AM
Pipette Filler
Points: 579, Level: 11 Points: 579, Level: 11 Points: 579, Level: 11
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2008
Posts: 12
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: cyclic peptide

As David mentioned that is depending on the number of disulfide bridges. Furthermore one should look at the sequence.

In general:
(a) 1 disulfide bridge:
Cys(Trt) and oxidation by air or DMSO (if that is not a problem to your purification system)
(b) 2 disulfide bridges:
Cys (Trt) and oxidation by air or DMSO (if that is not a problem to your purification system) for the first disulfide bridge, Cys(Acm) for the second disulfide bridge using iodine as reagent
(c) more disulfide bridges:
sequence depending (tBu is a third dimension, but one should think about different synthetic strategies, e.g. fragment condensation of cyclized peptides)

All is different of course if you want to synthesize larger amounts ...

Good luck

Andreas
Reply With Quote
Reply

Tags
cyclic , peptide


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Intramolecular cyclic thioether bond in Peptide Kunal Pandya Peptide Forum 10 08-19-2011 10:18 AM
disulfide cleavage dpar Peptide Forum 5 08-20-2010 01:09 PM
Proteins & Amino Acids/ Multiple Choice Check work please! SillyJilly Biochemistry Forum 0 02-23-2010 08:31 AM
96 well plate peptide synthesis service glbiochem Chit Chat 0 11-15-2007 05:22 AM
Website on Peptide Research and Suppliers Peptide Protein Forum 0 04-09-2006 07:50 PM


All times are GMT. The time now is 02:15 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13051 seconds with 16 queries