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| How many disulfide bond in your sequence? If only one, it would be quite easy. You can use Fmoc-Cys(Trt)-OH insert in synthesis, and oxidate in water solution. If over one, you need to choose Fmoc-Cys(Acm)-OH and Fmoc-Cys(tBu)-OH to oxidate and deprotect by different strategy. Mr. David Wei Mobile: 0086-15301650007(24 hour) Tel: 0086-21-61263307 Fax: 0086-21-61263399 E-mail: glbiochem@online.sh.cn |
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| As David mentioned that is depending on the number of disulfide bridges. Furthermore one should look at the sequence. In general: (a) 1 disulfide bridge: Cys(Trt) and oxidation by air or DMSO (if that is not a problem to your purification system) (b) 2 disulfide bridges: Cys (Trt) and oxidation by air or DMSO (if that is not a problem to your purification system) for the first disulfide bridge, Cys(Acm) for the second disulfide bridge using iodine as reagent (c) more disulfide bridges: sequence depending (tBu is a third dimension, but one should think about different synthetic strategies, e.g. fragment condensation of cyclized peptides) All is different of course if you want to synthesize larger amounts ... Good luck Andreas |
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