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making free base of a peptide

making free base of a peptide - Peptide Forum

making free base of a peptide - Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.


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  #1  
Old 03-03-2007, 04:51 AM
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Thumbs down making free base of a peptide



hello,
I would like to make a free base from HCl salt of a peptide. Please send your suggestions,
thank you for your help
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Old 12-21-2008, 10:22 AM
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Default Re: making free base of a peptide

Add little bit of Saturated Sodium Bicarbonate solution(Check each time pH before and after adding the base)

If you want to extract into the organic layer extract with Ethyl Acetate.Good Luck
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Old 02-05-2009, 05:21 AM
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Default Re: making free base of a peptide

Better to use free base resins for desalting of peptides
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Old 02-06-2009, 09:56 AM
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Default Re: making free base of a peptide

Quote:
Originally Posted by peptideraja View Post
Add little bit of Saturated Sodium Bicarbonate solution(Check each time pH before and after adding the base)

If you want to extract into the organic layer extract with Ethyl Acetate.Good Luck
i am doing dipeptide purification from lactobacillus please is ther any separation procedure for di peptide
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