Arginine Containing Peptides

jthundim · #1 (**permalink**) 02-22-2007, 11:17 PM

Forum members,
What would be the best way to make free base of an arginine (5 arginines)containing peptide (HCl salt).
Greatly appreciate your comments

thanks
jyo

glbiochem · #2 (**permalink**) 11-16-2007, 12:24 AM

Is it difficult? We had several such kind of peptides.
The cleavage of pbf is not easy, but their purification seems to be good.

I only met one peptide that contains 8 Arg and 1 Cys. The yield is very low.
I found this peptide was very easy oxidated.
From the LC-MS spec., I observed that the peptide formed inter-oxidation.

Biopep · #3 (**permalink**) 12-02-2007, 12:50 PM

Hi
Jthundim
I synthesized 8 arginine containing peptide with pmc protecting group. I use reagent K for cleavage.
Hope, this condition will work with you.

Regards

Bishnu Joshi

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Arginine Containing Peptides

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