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| Hello Tlaragio! Not sure exactly what you want to BLAST against. I have some programs that I used: virtual PCR (where you enter primer sequences) http://grup.cribi.unipd.it/cgi-bin/mateo/vpcr2.cgi If you have used Perl before, Perl Primer can BLAST primers you design in the program: http://perlprimer.sourceforge.net/tutorial.html For Linux (A Perl 5 interpreter or higher on a Linux system is required.), Oligo Picker BLASTs your primers in NCBI http://pga.mgh.harvard.edu/oligopicker/index.html Cheers! Last edited by moleculardude; 02-10-2007 at 08:52 AM. |
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