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Lid force knob on thermocycler won't budge!

Lid force knob on thermocycler won't budge! - PCR - Polymerase Chain Reaction Forum

Lid force knob on thermocycler won't budge! - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 06-16-2014, 08:44 PM
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Default Lid force knob on thermocycler won't budge!



Hi Friends,

A student forced one of our thermocyclers open the other day and now its lid force knob won't turn. In other words, we can load samples and close the lid, but we can't lower the bar that clamps down and prevents the tubes from opening.

I'm currently in touch with the manufacturer, but am afraid that it will cost a lot of money to get fixed. Just curious, has anyone else had this problem before and is there a way to fix it?

Thank you!
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Old 06-18-2014, 03:25 PM
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Default Re: Lid force knob on thermocycler won't budge!

It would be very useful if you post the model and the brand of your thermocycler, so that answer can be solved more directly.

I've never have this problem before (fortunately) but to my understanding the knob/lid is merely mechanic (gears and stuff), or at least that applies to labnet and eppendorf conventional thermocyclers (the ones I've used). Nevertheless, in order to fix it you will have to open the lid to see what's wrong with it first. Be very careful though, as the lid should have sensors and other electronic stuff that regulates temperature.

I wish you luck whatever you decide to do.
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Old 08-28-2014, 11:30 AM
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Default Re: Lid force knob on thermocycler won't budge!

I suppose there's the old mineral oil trick? Where you cover your samples with a layer of mineral oil to stop them evaporating?
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