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Cresol Red/Sucrose

Cresol Red/Sucrose - PCR - Polymerase Chain Reaction Forum

Cresol Red/Sucrose - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 06-26-2013, 07:45 PM
Pipette Filler
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Default Cresol Red/Sucrose



Hi.
Does anyone have experience using cresol red/sucrose as loading dye added to the reaction BEFORE PCR?
Does cresol red/sucrose have to have a certain pH? What are the tricks using it?

My cresol red reaction used to work but suddenly cresol red/sucrose seems to inhibit the PCR reaction... (I am sure it is due to cresol red/sucrose as I have already done some tests to exclude other possibilities of PCR failure...)

Thank you for any useful input.
Gala
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Old 07-01-2013, 09:14 AM
Pipette Filler
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Default Re: Cresol Red/Sucrose

My cresol red is supposed to run at 150bp; but now that it is not working anymore it is running at 75bp together with the primer dimers.
Does anyone have an explanation fo this? or has had something similar happen to you before?
Thanks for your input!
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