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-   -   Cresol Red/Sucrose (http://www.molecularstation.com/forum/pcr-polymerase-chain-reaction-forum/87957-cresol-red-sucrose.html)

gala_2 06-26-2013 07:45 PM

Cresol Red/Sucrose
 
Hi.
Does anyone have experience using cresol red/sucrose as loading dye added to the reaction BEFORE PCR?
Does cresol red/sucrose have to have a certain pH? What are the tricks using it?

My cresol red reaction used to work but suddenly cresol red/sucrose seems to inhibit the PCR reaction... (I am sure it is due to cresol red/sucrose as I have already done some tests to exclude other possibilities of PCR failure...)

Thank you for any useful input.
Gala :mf_surrender:

gala_2 07-01-2013 09:14 AM

Re: Cresol Red/Sucrose
 
My cresol red is supposed to run at 150bp; but now that it is not working anymore it is running at 75bp together with the primer dimers.
Does anyone have an explanation fo this? or has had something similar happen to you before?
Thanks for your input!


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