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mystery pcr

mystery pcr - PCR - Polymerase Chain Reaction Forum

mystery pcr - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 02-14-2013, 07:31 PM
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Default mystery pcr



hy

i extracted genomic DNA from blood in 2 ways:
1- frozen in EDTA tubes by Bioneer extraction kit.
2- fresh in EDTA tubes by Genaid extraction kit .

doing pcr to them:
1- few samples give product, the rest faild...i re extracted failed samples in the same way ,,no result in pcr,,only dimer.

2- in case the fresh blood ,good result.

is there a problem with genomic DNA.
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