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-   -   mystery pcr (http://www.molecularstation.com/forum/pcr-polymerase-chain-reaction-forum/87574-mystery-pcr.html)

may84 02-14-2013 07:31 PM

mystery pcr
 
hy

i extracted genomic DNA from blood in 2 ways:
1- frozen in EDTA tubes by Bioneer extraction kit.
2- fresh in EDTA tubes by Genaid extraction kit .

doing pcr to them:
1- few samples give product, the rest faild...i re extracted failed samples in the same way ,,no result in pcr,,only dimer.

2- in case the fresh blood ,good result.

is there a problem with genomic DNA.


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