gradient pcr trouble
hi.. i am facing troble with gradiant pcr. its siRNA viral construct of about 900 bps. i have pcr it on 55-65 many times it shows a light band on 63 but nothing at 64C and 62C. at 63 it also shows a couple of non-specific DNA bands..i ve changed taq, quantity of taq, pcr buffer (kcl & NH4) both tested, dNTPs and primers working fine. any solution????
|All times are GMT. The time now is 02:57 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved