| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
11-23-2012, 12:14 AM
| |||||||||||
| |||||||||||
 Relative gene expression analysis Hi everyone, I have a question about the analysis of results for relative gene expression. I'm looking to compare the relative expression levels of 3 different genes within the same sample and use GAPDH as the housekeeping gene (I don't have a reference sample). However, I have a large number of samples which means I'll be running 1 gene/plate. My question is can I set the same threshold level (the line used for Ct calculation) for all the genes? They are all taqman probes and have the same reporter dye. For example, if 1 plate with gene A has a threshold of 0.2, can I set the same 0.2 threshold for gene B and gene C in the other plates?  |
|
| Tags |
| analysis , expression , gene , relative |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Human Cytome Project - Update 24 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 1 | 08-01-2010 02:18 PM |
| New Saccharomyces Sequences 11/27/04 | Mike Cherry | Yeast Forum | 0 | 11-28-2004 11:39 PM |
| New Saccharomyces Sequences 09/08/04 | SGD Sequences | Yeast Forum | 0 | 09-13-2004 10:07 PM |
| New Saccharomyces Sequences 08/11/04 | SGD Sequences | Yeast Forum | 0 | 08-12-2004 12:26 AM |
| New Saccharomyces Sequences 05/19/04 | SGD Sequences | Yeast Forum | 0 | 05-23-2004 04:06 PM |
Linear Mode
