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#1
11-19-2012, 01:57 PM
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 qRT-PCR primer I am working with isoforms which have very similar sequences, so is it okay if I have same 5' primer for 2 genes and different 3' primer, similarly have same 3' primer for 2 genes and different 5' primer?  |
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#2
12-19-2012, 10:53 AM
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| Re: qRT-PCR primer [Only registered users see links. ] needs to primers, each complementary to a different strand, because the primers define the region that will be amplified. In the first cycle, the double stranded DNA is heated, so that the hydrogen bonds holding the 2 strands together break, and the 2 strands fall apart. When the temperature goes down, |
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| primer , qrtpcr |
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