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qRT-PCR primer

qRT-PCR primer - PCR - Polymerase Chain Reaction Forum

qRT-PCR primer - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 11-19-2012, 02:57 PM
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Default qRT-PCR primer



I am working with isoforms which have very similar sequences, so is it okay if I have same 5' primer for 2 genes and different 3' primer, similarly have same 3' primer for 2 genes and different 5' primer?
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Old 12-19-2012, 11:53 AM
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Default Re: qRT-PCR primer

[Only registered users see links. ] needs to primers, each complementary to a different strand, because the primers define the region that will be amplified. In the first cycle, the double stranded DNA is heated, so that the hydrogen bonds holding the 2 strands together break, and the 2 strands fall apart. When the temperature goes down,
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