I am working with isoforms which have very similar sequences, so is it okay if I have same 5' primer for 2 genes and different 3' primer, similarly have same 3' primer for 2 genes and different 5' primer?
Re: qRT-PCR primer
[Only registered and activated users can see links. Click Here To Register...] needs to primers, each complementary to a different strand, because the primers define the region that will be amplified. In the first cycle, the double stranded DNA is heated, so that the hydrogen bonds holding the 2 strands together break, and the 2 strands fall apart. When the temperature goes down,
|All times are GMT. The time now is 01:57 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved