qRT-PCR primer
 

I am working with isoforms which have very similar sequences, so is it okay if I have same 5' primer for 2 genes and different 3' primer, similarly have same 3' primer for 2 genes and different 5' primer?

Re: qRT-PCR primer
 

[Only registered and activated users can see links. Click Here To Register...] needs to primers, each complementary to a different strand, because the primers define the region that will be amplified. In the first cycle, the double stranded DNA is heated, so that the hydrogen bonds holding the 2 strands together break, and the 2 strands fall apart. When the temperature goes down,