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-   -   qRT-PCR primer (http://www.molecularstation.com/forum/pcr-polymerase-chain-reaction-forum/86834-qrt-pcr-primer.html)

rasing02 11-19-2012 02:57 PM

qRT-PCR primer
 
I am working with isoforms which have very similar sequences, so is it okay if I have same 5' primer for 2 genes and different 3' primer, similarly have same 3' primer for 2 genes and different 5' primer?

davidflora 12-19-2012 11:53 AM

Re: qRT-PCR primer
 
[Only registered and activated users can see links. Click Here To Register...] needs to primers, each complementary to a different strand, because the primers define the region that will be amplified. In the first cycle, the double stranded DNA is heated, so that the hydrogen bonds holding the 2 strands together break, and the 2 strands fall apart. When the temperature goes down,


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