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using low grade DMSO

using low grade DMSO - PCR - Polymerase Chain Reaction Forum

using low grade DMSO - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 07-26-2012, 07:42 PM
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Default using low grade DMSO



I have an issue with my current GC-rich PCR. The protocol requires that I use a high grade DMSO, which I believe I used (Sigma Hybri-Max DMSO). I haven't achieved much when using it. I did however try another DMSO that's labeled as "performance certified" or "cell-culture" certified. Whatever it is, I achieve much better results when using it. Unfortunately I don't have large quantities of it since I ordered 4 batches of the Hybri-Max DMSO seeing it being used in a previous protocol. I'm curious, is it possible to achieve the same quality of the "performance certified" DMSO if I increase the DMSO percentage of my current reaction with my lower grade DMSO. Has anyone tried this?
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