Storing PCR Product

[princeofnam]

i, I've unfortunately run a PCR that will finish passed the time the UV photo lab room closes. This PCR is simply an optimization run to see if the reaction works. I am thinking of storing the PCR product in the freezer and running a small amount on the gel I've already prepared. Will the gel I run tomorrow be of poorer quality? Or will storing it in the freezer result in practically similar results?

[PCR]

It will be fine in the freezer. I have ran PCR products on gels that have been left at room temp for days and they are still ok to visualize on a gel

[smhall]

I am seeing this 10 days since your post, so I am guessing you may have answers.

In cases like yours, I'd probably just leave the reaction tubes in the thermal cycler at 2-4 deg if it is < 12 hours from end of reaction to running on AGE/EtBr for the hour or so to visualize. I can't imagine the amplicons will be sorely affected.

[davidflora]

The problem with storing PCR products in water is if you have any nucleases around. It is better to store them in 10/0.1 TE. This way the pH won't drop from absorbance of CO2 and the EDTA will keep the nucleases from chewing up the DNA.[Only registered users see links. ]