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Storing PCR Product

Storing PCR Product - PCR - Polymerase Chain Reaction Forum

Storing PCR Product - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 07-02-2012, 10:12 PM
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Default Storing PCR Product



i, I've unfortunately run a PCR that will finish passed the time the UV photo lab room closes. This PCR is simply an optimization run to see if the reaction works. I am thinking of storing the PCR product in the freezer and running a small amount on the gel I've already prepared. Will the gel I run tomorrow be of poorer quality? Or will storing it in the freezer result in practically similar results?
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Old 07-09-2012, 06:21 AM
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Default Re: Storing PCR Product

It will be fine in the freezer. I have ran PCR products on gels that have been left at room temp for days and they are still ok to visualize on a gel
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Old 07-12-2012, 06:04 PM
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Default Re: Storing PCR Product

I am seeing this 10 days since your post, so I am guessing you may have answers.

In cases like yours, I'd probably just leave the reaction tubes in the thermal cycler at 2-4 deg if it is < 12 hours from end of reaction to running on AGE/EtBr for the hour or so to visualize. I can't imagine the amplicons will be sorely affected.
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Old 01-23-2013, 07:09 AM
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Default Re: Storing PCR Product

The problem with storing PCR products in water is if you have any nucleases around. It is better to store them in 10/0.1 TE. This way the pH won't drop from absorbance of CO2 and the EDTA will keep the nucleases from chewing up the DNA.[Only registered users see links. ]
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