First time posting on this website. I am a student researcher studying the effects of meth on various mRNA targets. I work in a psych lab that primarily has done behavioral neuroscience research and is trying to get into more molecular level diagnostics. After finding and establishing protocols for the lab, I have finally done my first PCR run, using a Bio-Rad iCycler iQ RT PCR Detection system....Basically it turned out terrible and I could use some help determining how to troubleshoot from here..
All input is much appreciated.