PCR on PCR products

[HernaicTom]

I have taken some PCR products and done another PCR on them, with the same primers but a different Taq.

I ran them on a gel, which shows bright bands of the desired size, but with some smearing.

Is this because there might simply be too much DNA as a result of the second PCR?

Does anyone know if I should avoid using these PCR products for cloning?

Thanks

[Dr. happy]

when u use A PCR - product as a template for the same reaction , the template may prime to itself creating such smear, so, if u have a good amplification with a smear u can gel - purify it or clean it up and use it for cloning..

[hineewinee]

I am trying to do a PCR using a PCR product. I used 2 µl as template. When I ran a gel of the PCR, I had a smear. What should I do?

[davidflora]

[Only registered users see links. ]Needing an maximum PCR response is to increase a particular locus without any unspecific by-products. Therefore, annealing needs to take place at a completely warm to allow only the most ideal DNA-DNA suits to happen in the response. For any given for beginners couple, the PCR system can be chosen depending on the composition