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PCR on PCR products

PCR on PCR products - PCR - Polymerase Chain Reaction Forum

PCR on PCR products - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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  #1  
Old 04-25-2012, 10:16 AM
Pipette Filler
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Default PCR on PCR products



I have taken some PCR products and done another PCR on them, with the same primers but a different Taq.

I ran them on a gel, which shows bright bands of the desired size, but with some smearing.

Is this because there might simply be too much DNA as a result of the second PCR?

Does anyone know if I should avoid using these PCR products for cloning?

Thanks
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Old 05-26-2012, 02:05 PM
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Default Re: PCR on PCR products

when u use A PCR - product as a template for the same reaction , the template may prime to itself creating such smear, so, if u have a good amplification with a smear u can gel - purify it or clean it up and use it for cloning..
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Old 07-31-2012, 07:58 AM
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Default Re: PCR on PCR products

I am trying to do a PCR using a PCR product. I used 2 Ál as template. When I ran a gel of the PCR, I had a smear. What should I do?
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Old 01-15-2013, 05:14 AM
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Default Re: PCR on PCR products

[Only registered users see links. ]Needing an maximum PCR response is to increase a particular locus without any unspecific by-products. Therefore, annealing needs to take place at a completely warm to allow only the most ideal DNA-DNA suits to happen in the response. For any given for beginners couple, the PCR system can be chosen depending on the composition
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