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How To Isolate DNA For The PCR Test Works

How To Isolate DNA For The PCR Test Works - PCR - Polymerase Chain Reaction Forum

How To Isolate DNA For The PCR Test Works - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 03-26-2012, 11:53 AM
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Default How To Isolate DNA For The PCR Test Works



PCR test comes in few test types. By this test you can analyze nucleic acids that are included in RNA or DNA. But DNA is more used in PCR tests because itís more stable than RNA and molecules in DAN can be isolated easier than these molecules in RNA. Only a single DNA simple is enough for a polymerase chain reaction test.

The most important step in polymerase chain reaction is to make it sure that your DNA you are going to test should not be contaminated with other DNAís and it have to be isolated very well. For isolating the DNA you can follow the steps bellow.

You can Obtain Cells that contain DNA from fingernails, swabbing in the inside walls of the mouth or from hair roots. See the forth stepÖ
Centrifuge these cells you obtained at 1200- 1500Xg for approximately 5 minutes. After this you can resuspend these cells in 1 ml PBS in the same centrifuge speed, 1200- 1500Xg for approximately 5 minutes. After repeating wash these cells in distilled water. In this step the only thing you must be aware is cell debrisís. if this occur see step four.
Now you can place the DNA container in boiling water again for approximately 5 min. this way you can deactivate the DNAse molecules that can be found in centrifuge and other preparation steps. After this concentrate the sample with ethanol alcohol and in this way the sample will be ready for PCR test.

Usually this kind of PCR test is done twice or 3 times to be sure and provides the control of the quality. And this test is important because mostly its used for HIV control.
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