I have troubles with the repeatability of my results on PCR. Both intensity and presence of the bands are everytime different. First I thought I made some mistakes preparing my master mix, so I did the same PCR's over and over again without any clear result... also my colleages appear to have some difficulties with the same primers and samples I use. Interestingly, till now it's only with specific primers of one specific gene that give an inconsequent result.
Has anyone an idea what is going wrong?